Arrival of the cord blood at our laboratory Cord blood bag with bar code and the according documentation Data of the cord blood and information on the documents are entered in the data base system Preparation of the closed bag system for the cryopreservation of the stem cells from the cord blood Application of the cord blood to the Sepax separation system Programming of the Sepax separation system Scanning the barcode of the bags before the separation Entering of the results obtained by the hematology analyser The Sepax machine system is starting to separate the stem cells from the cord blood Different plots for the determination of CD34+ cells and viability The closed bag system after the separation of the stem cells Preparation of the addition of cryoprotectant At this stage the cryoprotectant will be added to protect the stem cells during the freezing process After the separation process and the cryo protectant supplement, the stem cells are inserted in a metal box with individual bar codes Before the stem cells are finally stored they will be automatically frozen down to approximately -85 C° Once the stem cells have been frozen down they will be cryopreserved at -196 C° in the vapor phase of liquid nitrogen Finally the stem cells are "Saved for Life" The entrance of the new laboratory in Niel, Belgium
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